Anti-aging surgery is done which deal with the removal of aging and wrinkle effects on the human skin. Procedures make the person looks younger by reducing wrinkles, masking. Some non-surgical treatments for anti-wrinkles and anti-aging like Botox, facial dermal fillers, chemical peels, semi-permanent makeup. Estrogen stimulates fibroblasts to make collagen. Decreased levels of estrogen are associated with loss of collagen and increased wrinkling. Photoaging is also caused due to free radicals.